Application
OGDH antibody detects OGDH protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and blotted with OGDH antibody diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
OGDH antibody [C2C3], C-term detects OGDH protein at mitochondria on mouse muscle by immunohistochemical analysis. Sample: Paraffin-embedded mouse muscle. OGDH antibody [C2C3], C-term dilution: 1:500.Application
OGDH antibody [C2C3], C-term detects OGDH protein at Mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in -20℃ 100% MeOH for 5 min. Green: OGDH protein stained by OGDH antibody [C2C3], C-term diluted at 1:500. Blue: Hoechst 33343 staining.Application
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using OGDH antibody at 1:500 dilution.Application
Sample (50 µg of whole cell lysate) A: Mouse brain 7.5% SDS PAGE diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | OGDH antibody , C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to OGDH (oxoglutarate (alpha-ketoglutarate) dehydrogenase (lipoamide))This gene encodes one subunit of the 2-oxoglutarate dehydrogenase complex. This complex catalyzes the overall conversion of 2-oxoglutarate (alpha-ketoglutarate) to succinyl-CoA and CO(2) during the Krebs cycle. The protein is located in the mitocondrial matrix and uses thiamine pyrophosphate as a cofactor. A congential deficiency in 2-oxoglutarate dehydrogenase activity is believed to lead to hypotonia, metabolic acidosis, and hyperlactatemia. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human OGDH. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Xenopus laevis, Chicken, BovineRat (93%), Xenopus laevis (84%), Chicken (87%), Bovine (97%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | AKGDH antibody E1k antibody OGDC antibody OGDH antibody oxoglutarate dehydrogenase (succinyl-transferring) antibody oxoglutarate decarboxylase antibody OGDC-E1 antibody 2-oxoglutarate dehydrogenase complex component E1 antibody "2-oxoglutarate dehy |
|---|---|
| Molecular Weight(MW) | 116 kDa (note) (2) |
| Cellular Localization | Mitochondrion matrix |
Application
OGDH antibody detects OGDH protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and blotted with OGDH antibody diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
OGDH antibody [C2C3], C-term detects OGDH protein at mitochondria on mouse muscle by immunohistochemical analysis. Sample: Paraffin-embedded mouse muscle. OGDH antibody [C2C3], C-term dilution: 1:500.
Application
OGDH antibody [C2C3], C-term detects OGDH protein at Mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in -20℃ 100% MeOH for 5 min. Green: OGDH protein stained by OGDH antibody [C2C3], C-term diluted at 1:500. Blue: Hoechst 33343 staining.
Application
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using OGDH antibody at 1:500 dilution.
Application
Sample (50 µg of whole cell lysate) A: Mouse brain 7.5% SDS PAGE diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | 293T , A431 , HeLa , HepG2 , Mouse brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
