Application
FACS analysis of (A) HL-60 and (B) Jurkat cells using Orai1 antibody (green) or isotype control (black).Application
WB analysis of Jurkat (lanes 1 and 2) and HL-60 (lanes 3 and 4) lysates using Orai1 antibody at a dilution of 1:200 in the absence (lens 1 and 3) or presence (lens 2 and 4) of blocking peptide.| Product Name | Orai1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Orai1The protein encoded by this gene is a membrane calcium channel subunit that is activated by the calcium sensor STIM1 when calcium stores are depleted. This type of channel is the primary way for calcium influx into T-cells. Defects in this gene are a cause of immune dysfunction with T-cell inactivation due to calcium entry defect type 1 (IDTICED1). [provided by RefSeq, Sep 2011] |
| Immunogen | Peptide (C)KKQPGQPRPTSK, corresponding to amino acid residues 203-214 of human Orai1 (Accession Q96D31). 2nd extracellular loop. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| FACS:1:200~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ORAI calcium release-activated calcium modulator 1 CRACM1 ORAT1 ORAI1 TMEM142A |
|---|---|
| Target | Orai1 |
Application
FACS analysis of (A) HL-60 and (B) Jurkat cells using Orai1 antibody (green) or isotype control (black).
Application
WB analysis of Jurkat (lanes 1 and 2) and HL-60 (lanes 3 and 4) lysates using Orai1 antibody at a dilution of 1:200 in the absence (lens 1 and 3) or presence (lens 2 and 4) of blocking peptide.| Application Notes | FACS:1:200~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | The reconstituted solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C or below. Avoid multiple freezing and thawing. |
| Storage Buffer | phosphate buffered saline (PBS), pH 7.4, 1% BSA and 0.05% NaN3. |
