Application
Anti-Orai1_(extracellular) - Western blot analysis of rat spleen (lanes 1 and4), rat pancreas (lanes 2 and 5) and mouse B-cell lymphoma (WEHI) (lanes 3 and 6) lysates: 1-3. Anti-Orai1 (extracellular) antibody, (1:200). 4-6. Anti-Orai1 (extracellular) antibody, preincubated with the control peptide antigen.Application
Anti-Orai1_(extracellular) - Expression of Orai1 in rat RBL cells Immunocytochemical staining of intact living rat basophilic leukemia (RBL) cells. A. Extracellular staining of cells using Anti-Orai1 (extracellular) antibody, (1:100) followed by goat anti-rabbit-AlexaFluor-488 secondary antibody (green). B. Merge of A and Live view of the cells.Application
Anti-Orai1_(extracellular) - Expression of Orai1 in rat pancreas Immunohistochemical staining of rat pancreas paraffin embedded sections using Anti-Orai1 (extracellular) antibody, (1:50). Orai1 (brown) is expressed in acinar cells. Hematoxilin is used as the counterstain.Application
Anti-Orai1_(extracellular) - Indirect flow cytometry analysis of WEHI living cells: Black:Unstained cells. Green:Cells + Anti-Orai1 (extracellular) antibody, (10 μg/0.5 x 106 cells).| Product Name | Orai1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Orai1 |
| Immunogen | Peptide (C)KFLPLKRQAGQPS, corresponding to amino acid residues 200-212 of mouse Orai1 (Accession Q8BWG9 ). 2nd extracellular loop. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FACSICC/IFIHCIHC-PWB |
| FACS:1:200~1:500 ICC/IF:1:200~1:500 IHC:1:50~1:200 IHC-P:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | MouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Tmem142a ORAI calcium release-activated calcium modulator 1 orai-1 D730049H07Rik |
|---|---|
| Target | Orai1 |
| Tissue Specificity | Will not recognize human Orai1. |
Application
Anti-Orai1_(extracellular) - Western blot analysis of rat spleen (lanes 1 and4), rat pancreas (lanes 2 and 5) and mouse B-cell lymphoma (WEHI) (lanes 3 and 6) lysates: 1-3. Anti-Orai1 (extracellular) antibody, (1:200). 4-6. Anti-Orai1 (extracellular) antibody, preincubated with the control peptide antigen.
Application
Anti-Orai1_(extracellular) - Expression of Orai1 in rat RBL cells Immunocytochemical staining of intact living rat basophilic leukemia (RBL) cells. A. Extracellular staining of cells using Anti-Orai1 (extracellular) antibody, (1:100) followed by goat anti-rabbit-AlexaFluor-488 secondary antibody (green). B. Merge of A and Live view of the cells.
Application
Anti-Orai1_(extracellular) - Expression of Orai1 in rat pancreas Immunohistochemical staining of rat pancreas paraffin embedded sections using Anti-Orai1 (extracellular) antibody, (1:50). Orai1 (brown) is expressed in acinar cells. Hematoxilin is used as the counterstain.
Application
Anti-Orai1_(extracellular) - Indirect flow cytometry analysis of WEHI living cells: Black:Unstained cells. Green:Cells + Anti-Orai1 (extracellular) antibody, (10 μg/0.5 x 106 cells).| Application Notes | FACS:1:200~1:500 ICC/IF:1:200~1:500 IHC:1:50~1:200 IHC-P:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3. |
