Application
Immunohistochemical analysis of paraffin-embedded CL1-0 Xenograft, using ORC2 antibody at 1:100 dilution.Application
Sample (30 ug of whole cell lysate) A: Raji 7.5% SDS PAGE ORC2 antibody diluted at 1:1000| Product Name | ORC2 antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to ORC2 (origin recognition complex, subunit 2-like (yeast))The origin recognition complex (ORC) is a highly conserved six subunits protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. The protein encoded by this gene is a subunit of the ORC complex. This protein forms a core complex with ORC3L, -4L, and -5L. It also interacts with CDC45L and MCM10, which are proteins known to be important for the initiation of DNA replication. This protein has been demonstrated to specifically associate with the origin of replication of Epstein-Barr virus in human cells, and is thought to be required for DNA replication from viral origin of replication. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ORC2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, BovineMouse (84%), Rat (84%), Bovine (92%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ORC2L antibody ORC2 antibody origin recognition complex protein 2 homolog antibody "origin recognition complex subunit 2 homolog antibody" origin recognition complex subunit 2 antibody "origin recognition complex subunit 2 antibody" |
|---|---|
| Target | ORC2 |
| Molecular Weight(MW) | 66 kDa (note) |
| Cellular Localization | Nucleus |
Application
Immunohistochemical analysis of paraffin-embedded CL1-0 Xenograft, using ORC2 antibody at 1:100 dilution.
Application
Sample (30 ug of whole cell lysate) A: Raji 7.5% SDS PAGE ORC2 antibody diluted at 1:1000| Positive Control | Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
