Application
IHC-Fr analysis of mouse septum using Orexin Receptor 1 antibody at a dilution of 1:50 (green), Parvalbumin antibody (red) and Hoechst 33342 (blue). (C) Merge of A and B.Application
ICC/IF analysis of Colo-205 cells using Orexin Receptor 1 antibody at a dilution of 1:200 (red). (B) Live view of the same field as in (A). C. Nuclei were stained with Hoechst 33342 (blue).Application
IHC-Fr analysis of rat brainstem using Orexin Receptor 1 antibody at a dilution of 1:50 (red) and Hoechst 33342 (blue).Application
IHC-P analysis of rat colon using Orexin Receptor 1 antibody at a dilution of 1:100. M: mucosa. sm: submucosa. ME: muscularis externa.Application
WB analysis of Colo-205 (lanes 1 and 3) and HT-29 (lanes 2 and 4) lysates using Orexin Receptor 1 antibody at a dilution of 1:500 in the absence (lens 1 and 2) or presence (lens 3 and 4) of blocking peptide.Application
WB analysis of mouse brain lysates using Orexin Receptor 1 antibody at a dilution of 1:200 in the (1) absence or (2) presence of blocking peptide.Application
WB analysis of rat brain lysates using Orexin Receptor 1 antibody at a dilution of 1:500 in the (1) absence or (2) presence of blocking peptide.| Product Name | Orexin Receptor 1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Orexin Receptor 1neuropeptide receptor that binds orexin A; mediates orexin A modulation of the sleep-wake cycle [RGD, Feb 2006] |
| Immunogen | Peptide (C)RNWKRPSEQLEAQH, corresponding to amino acid residues 256-269 of rat OX1R (Accession P56718). 3rd intracellular loop. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIHC-FIHC-PWB |
| ICC/IF:1:200~1:500 IHC:1:50~1:200 IHC-F:1:50~1:100 IHC-P:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | hypocretin (orexin) receptor 1 Hcrtr1 Hctr1 |
|---|---|
| Target | Orexin Receptor 1 |
Application
IHC-Fr analysis of mouse septum using Orexin Receptor 1 antibody at a dilution of 1:50 (green), Parvalbumin antibody (red) and Hoechst 33342 (blue). (C) Merge of A and B.
Application
ICC/IF analysis of Colo-205 cells using Orexin Receptor 1 antibody at a dilution of 1:200 (red). (B) Live view of the same field as in (A). C. Nuclei were stained with Hoechst 33342 (blue).
Application
IHC-Fr analysis of rat brainstem using Orexin Receptor 1 antibody at a dilution of 1:50 (red) and Hoechst 33342 (blue).
Application
IHC-P analysis of rat colon using Orexin Receptor 1 antibody at a dilution of 1:100. M: mucosa. sm: submucosa. ME: muscularis externa.
Application
WB analysis of Colo-205 (lanes 1 and 3) and HT-29 (lanes 2 and 4) lysates using Orexin Receptor 1 antibody at a dilution of 1:500 in the absence (lens 1 and 2) or presence (lens 3 and 4) of blocking peptide.
Application
WB analysis of mouse brain lysates using Orexin Receptor 1 antibody at a dilution of 1:200 in the (1) absence or (2) presence of blocking peptide.
Application
WB analysis of rat brain lysates using Orexin Receptor 1 antibody at a dilution of 1:500 in the (1) absence or (2) presence of blocking peptide.| Application Notes | ICC/IF:1:200~1:500 IHC:1:50~1:200 IHC-F:1:50~1:100 IHC-P:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20oC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Reconstituted antibody containsA?phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3. |
