Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using OTC antibody at 1:250 dilution.Application
Sample (20 µg of whole cell lysate) A: mouse Liver 10% SDS PAGE diluted at 1:30000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample (30 µg of whole cell lysate) A: A431 12% SDS PAGE diluted at 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | OTC antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to OTC (ornithine carbamoyltransferase)This nuclear gene encodes a mitochondrial matrix enzyme. Missense, nonsense, and frameshift mutations in this enzyme lead to ornithine transcarbamylase deficiency, which causes hyperammonemia. Since the gene for this enzyme maps close to that for Duchenne muscular dystrophy, it may play a role in that disease also. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human OTC. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Pig, Chicken, BovineRat (93%), Pig (94%), Chicken (80%), Bovine (94%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | MGC129967 antibody MGC129968 antibody MGC138856 antibody OCTD antibody OTC antibody ornithine transcarbamylase antibody OTCase antibody "ornithine carbamoyltransferase mitochondrial antibody" ornithine carbamoyltransferase antibody |
|---|---|
| Molecular Weight(MW) | 40 kDa (note) |
| Cellular Localization | Mitochondrion matrix |
Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using OTC antibody at 1:250 dilution.
Application
Sample (20 µg of whole cell lysate) A: mouse Liver 10% SDS PAGE diluted at 1:30000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample (30 µg of whole cell lysate) A: A431 12% SDS PAGE diluted at 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | A431 , HepG2 , mouse Liver |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
