Application
C. Immunofluorescent staining of A431 cells using Phospho-specific anti-P120 Catenin RabMAb (cat. # ).Application
B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Phospho-specific anti-P120 Catenin RabMAb (cat. # ).Application
A. Western blot analysis on HeLa cell lysates using anti-Phospho-p120 Catenin (pS268) RabMAb (cat. #) 1:5000 dilution. Cells were either (A) untreated (B) treated with Calyculin A (cat. # ).| Product Name | p120 Catenin (phospho Ser268) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit monoclonal antibody [EPR2380] to p120 Catenin (phospho Ser268)Catenins are proteins that are linked to the cytoplasmic domain of transmembrane cadherins. p120 Catenin is one member of this Armadillo gene family of junctional plaque proteins . The association of catenins to cadherins produces a complex which is linked to the actin filament network, and which seems to be important for cadherins cell-adhesion properties. Recently it became clear that some catenins also mediate nuclear signaling . p120 Catenin is implicated both in cell transformation by SRC and in ligand-induced receptor signaling through the EGF, PDGF, CSF-1 and ERBB2 receptors. |
| Immunogen | A phospho-specific peptide corresponding to residues surrounding serine 268 of human p120 Catenin was used as an immunogen. |
| Modification | p-Ser268 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | EPR2380 |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| For WB: Use at a dilution of 1:2500 - 5000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | p120 P120CTN KIAA0384 CAS P120CAS CTNND1 CTNND p120(CAS) p120(CTN) p120 Catenin (phospho S268) p120 Catenin (phospho Ser268) p120 Catenin (pS268) p120 Catenin (pSer268) p120 Catenin phospho S268 p120 Catenin phospho Ser268 Phospho p120 Caten |
|---|---|
| Target | p120 Catenin Phospho (pS268) |
| Molecular Weight(MW) | 90 - 120 (note) |
| Tissue Specificity | The antibody only detects p120 Catenin phosphorylated on serine 268. |
Application
C. Immunofluorescent staining of A431 cells using Phospho-specific anti-P120 Catenin RabMAb (cat. # ).
Application
B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Phospho-specific anti-P120 Catenin RabMAb (cat. # ).
Application
A. Western blot analysis on HeLa cell lysates using anti-Phospho-p120 Catenin (pS268) RabMAb (cat. #) 1:5000 dilution. Cells were either (A) untreated (B) treated with Calyculin A (cat. # ).| Application Notes | For WB: Use at a dilution of 1:2500 - 5000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 °C. Stable for 12 months from date of receipt. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
