Application
Cytosolic fraction of rat brain extract (Lanes A, C) and ECV304 whole cell extract (Lane B) were separated on SDS-PAGE and blotted with Anti-p120ctn . The antibody was developed with Anti-Rabbit IgG, Peroxidase and chemiluminescent substrate. Lanes A, B: Antibody dilution 1:2,000 C: Antibody dilution 1:2,000 + 20 μg/ml p120ctn immunizing peptide| Product Name | p120 Catenin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to p120 Catenindelta 1 Catenin is an efficient tyrosine kinase substrate implicated both in cell transformation by SRC and in ligand-induced receptor signaling through the EGF, PDGF, CSF-1 and ERBB2 receptors. The association of catenins to cadherins produces a complex which is linked to the actin filament network, and which seems to be of primary importance for cadherins cell-adhesion properties. |
| Immunogen | Synthetic peptide conjugated to KLH, corresponding to amino acids 881-900 of Human delta 1 Catenin (isoform 1B). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| IF: Use at a dilution of 1/500. WB: Use at a dilution of 1/2000. Detects a band of approximately 120 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | delta1 Catenin 601045 P120CTN KIAA0384 CAS O60716 P120CAS delta 1 Catenin CTNND1 1500 CTNND |
|---|---|
| Target | p120 Catenin |
Application
Cytosolic fraction of rat brain extract (Lanes A, C) and ECV304 whole cell extract (Lane B) were separated on SDS-PAGE and blotted with Anti-p120ctn . The antibody was developed with Anti-Rabbit IgG, Peroxidase and chemiluminescent substrate. Lanes A, B: Antibody dilution 1:2,000 C: Antibody dilution 1:2,000 + 20 μg/ml p120ctn immunizing peptide| Positive Control | Rat brain extract, human endothelial ECV304 whole cell extract and human epidermoid carcinoma A431 cells |
|---|---|
| Application Notes | IF: Use at a dilution of 1/500. WB: Use at a dilution of 1/2000. Detects a band of approximately 120 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at −20°C |
| Storage Buffer | Preservative:15mM Sodium Azide; Constituents: 0.01M PBS (pH7.4) |
