Application
p130Cas (phospho Tyr165) antibody detects p130Cas (phospho Tyr165) protein at cell junction by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: p130Cas (phospho Tyr165) protein stained by p130Cas (phospho Tyr165) antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.Application
Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with p130Cas (phospho Tyr165) antibody diluted at 1:500. (CIP: Calf-intestinal alkaline phosphatase)| Product Name | p130Cas (phospho Tyr165) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to p130Cas (phospho Tyr165) (breast cancer anti-estrogen resistance 1)BCAR1, or CAS, is an Src (MIM 190090) family kinase substrate involved in various cellular events, including migration, survival, transformation, and invasion (Sawada et al., 2006 [PubMed 17129785]).[supplied by OMIM] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human p130Cas (phospho Tyr165). The exact sequence is proprietary. |
| Modification | p-Tyr165 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, RatMouse (100%), Rat (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | BCAR1 CAS1 CASS1 CAS P130Cas CRKAS breast cancer anti-estrogen resistance 1 |
|---|---|
| Target | p130Cas (phospho Tyr165) |
| Molecular Weight(MW) | 93 kDa (note) (7) |
| Cellular Localization | Cell junction , focal adhesion , Cytoplasm |
Application
p130Cas (phospho Tyr165) antibody detects p130Cas (phospho Tyr165) protein at cell junction by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: p130Cas (phospho Tyr165) protein stained by p130Cas (phospho Tyr165) antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Application
Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with p130Cas (phospho Tyr165) antibody diluted at 1:500. (CIP: Calf-intestinal alkaline phosphatase)| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
