Application
Western blot analysis of p130 Cas (pY249) expression in HEK293T EGF-treated (A); NIH3T3 EGF-treated (B); Raw264.7 EGF-treated (C); PC12 EGF-treated (D) whole cell lysates.Application
Immunofluorescent analysis of p130 Cas (pY249) staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).| Product Name | p130Cas (phospho Tyr249) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to p130Cas (phospho Tyr249)BCAR1, or CAS, is an Src (MIM 190090) family kinase substrate involved in various cellular events, including migration, survival, transformation, and invasion (Sawada et al., 2006 [PubMed 17129785]).[supplied by OMIM, May 2009] |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the center region of human p130 Cas. The exact sequence is proprietary. |
| Modification | p-Tyr249 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB: 1:500 - 1:1000. ICC/IF: 1:100 - 1:500: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | breast cancer anti-estrogen resistance 1 CASS1 CRKAS BCAR1 CAS CAS1 P130Cas |
|---|---|
| Target | p130Cas (phospho Tyr249) |
| Tissue Specificity | Recognizes endogenous levels of p130 Cas (pY249) protein. |
Application
Western blot analysis of p130 Cas (pY249) expression in HEK293T EGF-treated (A); NIH3T3 EGF-treated (B); Raw264.7 EGF-treated (C); PC12 EGF-treated (D) whole cell lysates.
Application
Immunofluorescent analysis of p130 Cas (pY249) staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).| Application Notes | WB: 1:500 - 1:1000. ICC/IF: 1:100 - 1:500: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4 °C. Upon delivery aliquot and store at -20 °C for one year. Avoid freeze/thaw cycles. |
| Storage Buffer | Liquid in 0.42% Potassium phosphate; 0.87% Sodium chloride; pH 7.3; 30% glycerol; and 0.01% sodium azide. |
