Application
Western blot analysis of extracts from Hela cells using p21Cip1(Ab-145) Antibody and the same antibody preincubated with blocking peptide| Product Name | p21 Cip1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to p21 Cip1May be the important intermediate by which p53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. |
| Immunogen | Peptide sequence around aa.143~147 (R-Q-T-S-M) derived from Human p21Cip1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CAP20 CDKN1 CIP1 MDA-6 P21 SDI1 WAF1 p21CIP1 CDKN1A cyclin-dependent kinase inhibitor 1 melanoma differentiation associated protein 6 wild-type p53-activated fragment 1 DNA synthesis inhibitor CDK-interacting protein 1 CDK-interaction protein |
|---|---|
| Target | p21 Cip1 |
| Molecular Weight(MW) | 21 (note) |
Application
Western blot analysis of extracts from Hela cells using p21Cip1(Ab-145) Antibody and the same antibody preincubated with blocking peptide| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
