Application
Western Blot: p27 KIP 1 Antibody - Western blot (WB) analysis of p27 KIP 1 (S10) antibody in extracts from COLO205 cells.| Product Name | p27 Kip1 (phospho Ser10) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to p27 Kip1 (phospho Ser10)This gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Mutations in this gene are associated with multiple endocrine neoplasia type IV (MEN4). [provided by RefSeq, Apr 2014] |
| Immunogen | Synthetic phosphopeptide derived from human p27 Kip1 around the phosphorylation site of Serine 10. |
| Modification | p-Ser10 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: 1:500 - 1:1000: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CDKN4 CDKN1B cyclin-dependent kinase inhibitor 1B (p27 Kip1) P27KIP1 KIP1 MEN1B MEN4 |
|---|---|
| Target | p27 Kip1 (phospho Ser10) |
Application
Western Blot: p27 KIP 1 Antibody - Western blot (WB) analysis of p27 KIP 1 (S10) antibody in extracts from COLO205 cells.| Application Notes | WB: 1:500 - 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate buffered saline with 0.05% Sodium Azide, pH 7.2. |
