Application
The anti-Phospho-p27Kip1-S10 Pab is used in Western blot to detect Phospho-p27Kip1-S10 in HL60 tissue lysateApplication
Formalin-fixed and paraffin-embedded human skeletal muscle with Phospho-p27Kip1-S10 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | p27 Kip1 (phospho Ser10) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to p27 Kip1 (phospho Ser10)This gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Mutations in this gene are associated with multiple endocrine neoplasia type IV (MEN4). [provided by RefSeq, Apr 2014] |
| Immunogen | Synthetic phosphopeptide corresponding to amino acid residues surrounding S10 of human p27Kip1 |
| Modification | p-Ser10 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| WB: 1:1000. IHC-P: 1:50 - 1:100: |
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| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | P27KIP1 KIP1 cyclin-dependent kinase inhibitor 1B (p27 Kip1) CDKN4 MEN4 MEN1B CDKN1B |
|---|---|
| Target | p27 Kip1 (phospho Ser10) |
| Tissue Specificity | This p27Kip1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S10 of human p27Kip1. |
Application
The anti-Phospho-p27Kip1-S10 Pab is used in Western blot to detect Phospho-p27Kip1-S10 in HL60 tissue lysate
Application
Formalin-fixed and paraffin-embedded human skeletal muscle with Phospho-p27Kip1-S10 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB: 1:1000. IHC-P: 1:50 - 1:100: |
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| Form | Liquid |
|---|---|
| Storage Instructions | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. |
