Application
IHC-P analysis of human breast carcinoma tissue using p27 Kip1 (phospho Thr187) antibody.Application
WB analysis of lysates from (1) untreated, (2) EGF-treated and (3) IFN gamma-treated HeLa cells using p27 Kip1 (phospho Thr187) antibody.| Product Name | p27 Kip1 (phospho Thr187) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to p27 Kip1 (phospho Thr187)This gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. [provided by RefSeq, Jul 2008] |
| Immunogen | The antiserum was produced against synthesized phosphopeptide derived from human p27Kip1 around the phosphorylation site of threonine 187. |
| Modification | p-Thr187 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| WB: 1:500-1:1000 IHC: 1:50-1:100: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | cyclin-dependent kinase inhibitor 1B (p27 Kip1) P27KIP1 CDKN1B KIP1 MEN1B MEN4 CDKN4 |
|---|---|
| Target | p27 Kip1 (phospho Thr187) |
| Tissue Specificity | This antibody detects endogenous levels of p27Kip1 only when phosphorylated at threonine 187. |
Application
IHC-P analysis of human breast carcinoma tissue using p27 Kip1 (phospho Thr187) antibody.
Application
WB analysis of lysates from (1) untreated, (2) EGF-treated and (3) IFN gamma-treated HeLa cells using p27 Kip1 (phospho Thr187) antibody.| Application Notes | WB: 1:500-1:1000 IHC: 1:50-1:100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C or -80°C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
