Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p27Kip1 (Phospho-Thr187) Antibody (left) or the same antibody preincubated with blocking peptide (right).Application
Western blot analysis of extracts from Hela cells untreated or treated with EGF, IFN- alpha using p27Kip1 (Phospho-Thr187) Antibody .| Product Name | p27 Kip1 (phospho Thr187) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to p27 Kip1 (phospho Thr187)Important regulator of cell cycle progression. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Positive regulator of cyclin D-dependent kinases such as CDK4. Regulated by phosphorylation and degradation events. |
| Immunogen | Peptide sequence around phosphorylation site of threonine 187 (E-Q-T(p)-P-K) derived from Human p27Kip1. |
| Modification | p-Thr187 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CDKN4 KIP1 MEN1B MEN4 P27KIP1 CDKN1B cyclin-dependent kinase inhibitor 1B cyclin-dependent kinase inhibitor 1B (p27 Kip1) |
|---|---|
| Target | p27Kip1 (Phospho Thr187) |
| Molecular Weight(MW) | 27 (note) |
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p27Kip1 (Phospho-Thr187) Antibody (left) or the same antibody preincubated with blocking peptide (right).
Application
Western blot analysis of extracts from Hela cells untreated or treated with EGF, IFN- alpha using p27Kip1 (Phospho-Thr187) Antibody .| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20oC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
