Application
Application| Product Name | p27 Kip1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to p27Cell cycle progression is regulated by cyclins and their cognate Cdks. p27 KIP 1 is a cell cycle regulatory mitotic inhibitor of cdk activity. p27 KIP 1 is a candidate tumor suppressor gene, and has been proposed to function as a possible mediator of TGF beta induced G1 arrest. p27 KIP 1 is up-regulated in response to antimitogenic stimuli. The increased protein expression of p27 results in cellular arrest by binding to cyclin/Cdk complexes such as cyclin D1/Cdk4. p27 Kip1 is regulated by phosphorylation on serine 10 (S10) and threonine 187 (T187). Phosphorylation by CDK2 on T187 results in ubiquitylation and degradation of p27 Kip 1; while phosphorylation by hKIS on S10 signals the nuclear export to the cytoplasm. |
| Immunogen | Synthetic peptide corresponding to C terminal of Human p27. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCIHC-PIPWB |
| IHC-P: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Detects a band of approximately 27 kDa (predicted molecular weight: 22 kDa). Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.2mg/ml |
| Gene Synonyms | P27KIP1 p27 KIP1 CDKN1B KIP1 1027 600778 P46527 CDKN4 |
|---|---|
| Target | p27 KIP1 |
| Tissue Specificity | Reacts with p27 (also designated Kip1 p27) |
| Cellular Localization | Nuclear and cytoplasmic in quiescent cells |
Application
Application| Positive Control | Jurkat, HeLa, NIH/3T3, and KNRK |
|---|---|
| Application Notes | IHC-P: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Detects a band of approximately 27 kDa (predicted molecular weight: 22 kDa). Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | PBS containing 0.1 % sodium azide and 0.2% gelatin |
