Application
Western blot analysis indicated that concatamerized trimers remain intact. Total protein from oocytes injected with RNA for wild type P2X2 monomer, or trimer HH-HH-HH, HA-AA-AH, and AA-AA-HH was separated by SDS-PAGE under reducing conditions on a 4–12% gradient gel along with protein from uninjected| Product Name | P2X2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to P2X2The product of this gene belongs to the family of purinoceptors for ATP. This receptor functions as a ligand-gated ion channel. Binding to ATP mediates synaptic transmission between neurons and from neurons to smooth muscle. Six transcript variants encoding six distinct isoforms have been identified for this gene. |
| Immunogen | Synthetic peptide: DSTSTDPKGLAQL, corresponding to amino acids 460-472 of Rat P2X2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-FWB |
| ICC: Use at a dilution of 1/1000. IHC-Fr: Use at a dilution of 1/1000. WB: Use at a dilution of 1/1000. Predicted molecular weight: 47,8 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMousePigRatGuinea PigPrimate |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | P2X2 |
|---|---|
| Target | P2X2 |
| Cellular Localization | Plasma membrane |
Application
Western blot analysis indicated that concatamerized trimers remain intact. Total protein from oocytes injected with RNA for wild type P2X2 monomer, or trimer HH-HH-HH, HA-AA-AH, and AA-AA-HH was separated by SDS-PAGE under reducing conditions on a 4–12% gradient gel along with protein from uninjected| Application Notes | ICC: Use at a dilution of 1/1000. IHC-Fr: Use at a dilution of 1/1000. WB: Use at a dilution of 1/1000. Predicted molecular weight: 47,8 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.05% Sodium Azide; Constituents: Whole Serum |
