Application
Sample (30 µg of whole cell lysate) A: MCF-7 10% SDS PAGE diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunohistochemical analysis of paraffin-embedded PC14 xenograft, using P2X3 antibody at 1:500 dilution.Application
Immunofluorescence analysis of methanol-fixed MCF-7, using P2X3 antibody at 1:500 dilution.| Product Name | P2X3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to P2X3 (purinergic receptor P2X, ligand-gated ion channel, 3)The product of this gene belongs to the family of purinoceptors for ATP. This receptor functions as a ligand-gated ion channel and may transduce ATP-evoked nociceptor activation. Mouse studies suggest that this receptor is important for peripheral pain responses, and also participates in pathways controlling urinary bladder volume reflexes. It is possible that the development of selective antagonists for this receptor may have a therapeutic potential in pain relief and in the treatment of disorders of urine storage. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human P2X3. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, RatMouse (94%), Rat (94%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | MGC129956 antibody P2X3 antibody P2RX3 antibody P2X purinoceptor 3 antibody "P2X receptor subunit 3 antibody" purinoceptor P2X3 antibody ATP receptor antibody purinergic receptor P2X3 antibody "purinergic receptor P2X ligand-gated ion channel 3 |
|---|---|
| Molecular Weight(MW) | 44 kDa (note) (2) |
| Cellular Localization | Membrane; Multi-pass membrane protein |
Application
Sample (30 µg of whole cell lysate) A: MCF-7 10% SDS PAGE diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunohistochemical analysis of paraffin-embedded PC14 xenograft, using P2X3 antibody at 1:500 dilution.
Application
Immunofluorescence analysis of methanol-fixed MCF-7, using P2X3 antibody at 1:500 dilution.| Positive Control | NT2D1 , IMR32 , U87-MG , MCF-7 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
