Application
Anti-P2X7_Receptor_(extracellular) - Western blot analysis of rat brain membranes (lanes 1,5) and human cell lines; K562 (lanes 2 and 6); WEHI-231 (lanes 3 and 7) and HL-60 (lanes 4 and 8): 1-4. Anti-P2X7 Receptor (extracellular) antibody, (1:200). 5-8. Anti-P2X7 Receptor (extracellular) antibody, preincubated with the control peptide antigen.Application
Anti-P2X7_Receptor_(extracellular) - Expression of P2X7 in rat pancreas Immunohistochemical staining of rat pancreas frozen section using Anti-P2X7 Receptor (extracellular) antibody, (1:100), followed by mouse anti-rabbit-AlexaFluor-594 secondary antibody (red). Staining is present in endocrine cells of the Isle of Langerhans. Hoechst 33342 is used as the counterstain.Application
Anti-P2X7_Receptor_(extracellular) - Expression of P2X7 in rat RBL cells Immunocytochemical staining of intact living rat basophilic leukemia (RBL) cells. A. Extracellular staining of cells using Anti-P2X7 Receptor (extracellular) antibody, (1:100) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Live view of the cells.| Product Name | P2X7 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to P2X7 |
| Immunogen | Peptide KKGWMDPQSKGIQTGRC, corresponding to amino acid residues 136-152 of mouse P2X7 Receptor (Accession number Q9Z1M0). Extracellular loop. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FACSICC/IFIHC-FWB |
| Recommended Starting Dilutions:
For WB: Use at a dilution of 1:200.
For IHC-Fr: Use at a dilution of 1:100.
For ICC/IF: Use at a dilution of 1:100.
For FACS: Use 5-10 µg antibody/1x10^6 cells.
Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | purinergic receptor P2X ligand-gated ion channel 7 P2X7R AI467586 P2rx7 P2X(7) |
|---|---|
| Target | P2X7 |
Application
Anti-P2X7_Receptor_(extracellular) - Western blot analysis of rat brain membranes (lanes 1,5) and human cell lines; K562 (lanes 2 and 6); WEHI-231 (lanes 3 and 7) and HL-60 (lanes 4 and 8): 1-4. Anti-P2X7 Receptor (extracellular) antibody, (1:200). 5-8. Anti-P2X7 Receptor (extracellular) antibody, preincubated with the control peptide antigen.
Application
Anti-P2X7_Receptor_(extracellular) - Expression of P2X7 in rat pancreas Immunohistochemical staining of rat pancreas frozen section using Anti-P2X7 Receptor (extracellular) antibody, (1:100), followed by mouse anti-rabbit-AlexaFluor-594 secondary antibody (red). Staining is present in endocrine cells of the Isle of Langerhans. Hoechst 33342 is used as the counterstain.
Application
Anti-P2X7_Receptor_(extracellular) - Expression of P2X7 in rat RBL cells Immunocytochemical staining of intact living rat basophilic leukemia (RBL) cells. A. Extracellular staining of cells using Anti-P2X7 Receptor (extracellular) antibody, (1:100) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Live view of the cells.| Application Notes | Recommended Starting Dilutions:
For WB: Use at a dilution of 1:200.
For IHC-Fr: Use at a dilution of 1:100.
For ICC/IF: Use at a dilution of 1:100.
For FACS: Use 5-10 µg antibody/1x10^6 cells.
Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3. |
