Application
Anti-P2Y11_Receptor - Western blot analysis human platelet lysates: 1. Anti-P2Y11 Receptor antibody, (1:200). 2. Anti-P2Y11 Receptor antibody, preincubated with the control peptide antigen.Application
Anti-P2Y11_Receptor - Expression of P2Y11 receptor in human P2Y11-MDCK transfected cells Immunocytochemical staining of paraformaldehyde-fixed and permeabilized human P2Y11-MDCK transfected cells. A. Staining using Anti-P2Y11 Receptor antibody, (1:200), followed by Alexa-488-conjugated goat-anti-rabbit secondary antibody. B. Nuclear fluorescence staining of cells using the membrane-permeable DNA dye Hoechst 33342. C. Merged images of panels A and B.| Product Name | P2Y11 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to P2Y11The product of this gene belongs to the family of G-protein coupled receptors. This family has several receptor subtypes with different pharmacological selectivity, which overlaps in some cases, for various adenosine and uridine nucleotides. This receptor is coupled to the stimulation of the phosphoinositide and adenylyl cyclase pathways and behaves as a selective purinoceptor. Naturally occuring read-through transcripts, resulting from intergenic splicing between this gene and an immediately upstream gene (PPAN, encoding peter pan homolog), have been found. The PPAN-P2RY11 read-through mRNA is ubiquitously expressed and encodes a fusion protein that shares identity with each individual gene product. [provided by RefSeq, Jul 2008] |
| Immunogen | Peptide (C)NATAAPKPSEPQSRELS, corresponding to amino acid residues 357-373 of human P2Y11 (Accession Q96G91). Intracellular, C-terminus. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FACSICC/IFWB |
| FACS:1:200~1:500 ICC/IF:1:200~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | purinergic receptor P2Y G-protein coupled 11 P2RY11 |
|---|---|
| Target | P2Y11 |
Application
Anti-P2Y11_Receptor - Western blot analysis human platelet lysates: 1. Anti-P2Y11 Receptor antibody, (1:200). 2. Anti-P2Y11 Receptor antibody, preincubated with the control peptide antigen.
Application
Anti-P2Y11_Receptor - Expression of P2Y11 receptor in human P2Y11-MDCK transfected cells Immunocytochemical staining of paraformaldehyde-fixed and permeabilized human P2Y11-MDCK transfected cells. A. Staining using Anti-P2Y11 Receptor antibody, (1:200), followed by Alexa-488-conjugated goat-anti-rabbit secondary antibody. B. Nuclear fluorescence staining of cells using the membrane-permeable DNA dye Hoechst 33342. C. Merged images of panels A and B.| Application Notes | FACS:1:200~1:500 ICC/IF:1:200~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3. |
