Application
Western blot analysis of extracts from 0.5 hr (Left lane) and 1 hr (Right lane) UV treated 3T3 cells.WB
Effects of total saponins from Dioscorea nipponica Makino and indomethacin on the protein levels of c-Jun, p-c-Jun, MAPK phosphatase, p-MKP, vascular cell adhesion molecule 1, intercellular adhesion molecule 1, C-X-C motif chemokine ligand 1, peroxisome proliferator-activated receptor γ, and Adipor2. All the groups were induced with IL-1β (10 µg/L), while both the total saponins from Dioscorea nipponica Makino (100 µg/L) and indomethacin (100 µg/L) groups were incubated for 72 hours.| Product Name | p38 MAPK (phospho Thr180/Tyr182) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to p38 MAPK (phospho Thr180/Tyr182)p38 MAP kinase is the mammalian homologue of the yeast HOG kinase and participates in a cascade controlling cellular responses to cytokines and stress. Like the SAPK/JNK pathway, p38 MAP kinase is activated by a variety of cellular stresses including inflammatory cytokines, UV light and growth factors, etc. Activated p38 MAP kinase has been shown to phosphorylate and activate MAPKAP kinase-2 and to phosphorylate the transcription factors ATF-2 and Max. |
| Modification | p-Thr180/Tyr182 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCIPWB |
| Western blotting (0.5-4 μg/ml), immunoprecipitation (10-20 mg/ml) and Immunohistochemistry (10-20 μg/ml). However, the optimal concentrations should be determined individually. The antibody recognizes 43 kDa phosphorylated p38 (Thr180/Tyr182) MAP kinase of human, mouse, and rat origins. Reactivity to other species has not been tested.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | stressactivated protein kinase 2A p38-MAPK p38 MAPK14 MAXinteracting protein2 stressactivated protein kinase2A stress-activated protein kinase 2A Mitogen Activated Protein Kinase14 RK phospho-p38 MAPK (pThr180/Tyr182) Mitogen Activated Protein Ki |
|---|---|
| Target | p38 MAPK (phospho Thr180/Tyr182) |
| Tissue Specificity | This antibody is specific for p38 MAPK (phospho Thr180/Tyr182). |
Application
Western blot analysis of extracts from 0.5 hr (Left lane) and 1 hr (Right lane) UV treated 3T3 cells.
WB
Effects of total saponins from Dioscorea nipponica Makino and indomethacin on the protein levels of c-Jun, p-c-Jun, MAPK phosphatase, p-MKP, vascular cell adhesion molecule 1, intercellular adhesion molecule 1, C-X-C motif chemokine ligand 1, peroxisome proliferator-activated receptor γ, and Adipor2. All the groups were induced with IL-1β (10 µg/L), while both the total saponins from Dioscorea nipponica Makino (100 µg/L) and indomethacin (100 µg/L) groups were incubated for 72 hours.| Application Notes | Western blotting (0.5-4 μg/ml), immunoprecipitation (10-20 mg/ml) and Immunohistochemistry (10-20 μg/ml). However, the optimal concentrations should be determined individually. The antibody recognizes 43 kDa phosphorylated p38 (Thr180/Tyr182) MAP kinase of human, mouse, and rat origins. Reactivity to other species has not been tested.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. For long term storage, aliquot and freeze at -70°C. Avoid repeated freeze/defrost cycles. |
| Storage Buffer | PBS containing 50% glycerol, 1% BSA, and 0.02% thimerosal |
