Application
A. Western blot analysis on HeLa cell lysate using anti-Phospho-p38 MAPK (ERK1) (pT180/pY182) RabMAb dilution 1:1000. Cells were either (1) untreated or (2) treated with Anisomycin.Application
B. Dot Blot analysis on immunogen phospho-peptide (A) and non-phospho peptide (B) using anti-Phospho-p38 MAPK (ERK1) (pT180/pY182) RabMAb dilution 1:1000.| Product Name | p38 MAPK (phospho Thr180/Tyr182) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit monoclonal antibody [E229] to p38 MAPK (phospho Thr180/Tyr182)p38 MAP kinase is a mitogen-activated protein kinase that functions in various intracellular signaling cascades controlling cellular stress responses. p38 MAP kinase is activated by a variety of cellular stresses including osmotic shock, inflammatory cytokines, lipopolysaccharides (LPS), UV light and growth factors . MKK3 and SEK activate p38 MAP kinase by phosphorylation at adjacent threonine and tyrosine residues, Thr180 and Tyr182 . Activated p38 MAP kinase phosphorylates numerous downstream targets, including transcription factor ATF-2 . |
| Immunogen | A synthetic phospho-peptide corresponding to residues surrounding Thr180 and Tyr182 of human p38 MAPK was used as immunogen. |
| Conjugation | Agarose |
| Modification | p-Thr180/Tyr182 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | E229 |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIPWB |
| WB:1:500~1:2000 IP:1:100~1:500 ICC/IF:1:50~1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | p38 MAPK14 PRKM15 PRKM14 RK CSBP1 SAPK2A CSPB1 Mxi2 p38ALPHA EXIP CSBP2 CSBP p38 MAPK (phospho T180/Y182) p38 MAPK (phospho Thr180/Tyr182) p38 MAPK (pT180/pY182) p38 MAPK (pThr180/pTyr182) p38 MAPK phospho T180/Y182 p38 MAPK phospho Thr18 |
|---|---|
| Target | p38 MAPK (phospho Thr180/Tyr182) |
| Molecular Weight(MW) | 42 (note) |
| Tissue Specificity | The antibody only detects p38 MAPK phosphorylated on Threonine 180 and Tyrosine 182. |
Application
A. Western blot analysis on HeLa cell lysate using anti-Phospho-p38 MAPK (ERK1) (pT180/pY182) RabMAb dilution 1:1000. Cells were either (1) untreated or (2) treated with Anisomycin.
Application
B. Dot Blot analysis on immunogen phospho-peptide (A) and non-phospho peptide (B) using anti-Phospho-p38 MAPK (ERK1) (pT180/pY182) RabMAb dilution 1:1000.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 ICC/IF:1:50~1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 °C. Stable for 12 months from date of receipt. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
