Application
Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer tissue sections using Anti-Phospho-p38 MAPK (Thr180/Tyr182) RM243 at a 1:1000 dilution.Application
Western Blot of HeLa cell lysates, treated or non-treated with anisomycin using Anti-Phospho-p38 MAPK (Thr180/Tyr182) RM243 at a 1:2000 dilution, showed a band of Phospho-p38 MAPK (~38 kDa) in anisomycin-treated HeLa cells.| Product Name | p38 MAPK (phospho Thr180/Tyr182) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Monoclonal antibody [RM243] to p38 MAPK (phospho Thr180/Tyr182)The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008] |
| Immunogen | A phospho-peptide corresponding to human Phospho-p38 MAPK (Thr180/Tyr182) |
| Modification | p-Thr180/Tyr182 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | RM243 |
| Host Species | Rabbit |
| Tested Applications | IHCIHC-PWB |
| Immunohistochemistry (IHC): 1:1000-1:2000 dilution, Western Blot (WB): 1:500-1:1000 dilution.: |
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| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | PRKM15 CSPB1 CSBP1 CSBP2 PRKM14 mitogen-activated protein kinase 14 p38ALPHA Mxi2 SAPK2A EXIP MAPK14 RK CSBP p38 |
|---|---|
| Target | p38 MAPK (phospho Thr180/Tyr182) |
| Tissue Specificity | This antibody reacts to p38 MAPK only when dual phosphorylation at Thr180 and Tyr182 is observed. There is no cross-reactivity with p38 MAPK without dual phosphorylation at Thr180 and Tyr182. |
Application
Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer tissue sections using Anti-Phospho-p38 MAPK (Thr180/Tyr182) RM243 at a 1:1000 dilution.
Application
Western Blot of HeLa cell lysates, treated or non-treated with anisomycin using Anti-Phospho-p38 MAPK (Thr180/Tyr182) RM243 at a 1:2000 dilution, showed a band of Phospho-p38 MAPK (~38 kDa) in anisomycin-treated HeLa cells.| Application Notes | Immunohistochemistry (IHC): 1:1000-1:2000 dilution, Western Blot (WB): 1:500-1:1000 dilution.: |
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| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20.0°C |
| Storage Buffer | 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
