Application
WB analysis of lysates from HeLa cells treated with TNFa or calf intestinal phosphatase (CIP) using p38 MAPK (phospho Tyr182) antibody.Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using P38 MAPK (Phospho-Tyr182) Antibody (left) or the same antibody preincubated with blocking peptide (right).Application
Western blot analysis of extracts from Hela cells untreated or treated with UV for the indicated times, using P38 MAPK(Phospho-Tyr182) AntibodyApplication
Western blot analysis of extracts from Hela cells untreated or treated with UV for the indicated times, using P38 MAPK (Phospho-Tyr182) Antibody .| Product Name | p38 MAPK (phospho Tyr182) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to p38 MAPK (phospho Tyr182)Responds to activation by environmental stress, pro-inflammatory cytokines and lipopolysaccharide (LPS) by phosphorylating a number of transcription factors, such as ELK1 and ATF2 and several downstream kinases, such as MAPKAPK2 and MAPKAPK5. Plays a critical role in the production of some cytokines, for example IL-6. May play a role in stabilization of EPO mRNA during hypoxic stress. Isoform Mxi2 activation is stimulated by mitogens and oxidative stress and only poorly phosphorylates ELK1 and ATF2. Isoform Exip may play a role in the early onset of apoptosis. |
| Immunogen | Peptide sequence around phosphorylation site of tyrosine 182 (T-G-Y(p)-V-A) derived from Human P38 MAPK. |
| Modification | p-Tyr182 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: . Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CSBP CSBP1 CSBP2 CSPB1 EXIP Mxi2 PRKM14 PRKM15 RK SAPK2A p38 p38ALPHA MAPK14 mitogen-activated protein kinase 14 Csaids binding protein MAP kinase Mxi2 cytokine suppressive anti-inflammatory drug-binding protein mitogen-activated protein k |
|---|---|
| Target | p38 MAPK (phospho Tyr182) |
| Molecular Weight(MW) | 43 (note) |
Application
WB analysis of lysates from HeLa cells treated with TNFa or calf intestinal phosphatase (CIP) using p38 MAPK (phospho Tyr182) antibody.
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using P38 MAPK (Phospho-Tyr182) Antibody (left) or the same antibody preincubated with blocking peptide (right).
Application
Western blot analysis of extracts from Hela cells untreated or treated with UV for the indicated times, using P38 MAPK(Phospho-Tyr182) Antibody
Application
Western blot analysis of extracts from Hela cells untreated or treated with UV for the indicated times, using P38 MAPK (Phospho-Tyr182) Antibody .| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: . Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
