Application
Western blot analysis of extracts from 293 and Hela cells using P38 MAPK Antibody .Application
Western blot analysis of extracts from 293 and Hela cells using P38 MAPK (Ab-182) Antibody| Product Name | P38 MAPK antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to P38 MAPKResponds to activation by environmental stress, pro-inflammatory cytokines and lipopolysaccharide (LPS) by phosphorylating a number of transcription factors, such as ELK1 and ATF2 and several downstream kinases, such as MAPKAPK2 and MAPKAPK5. Plays a critical role in the production of some cytokines, for example IL-6. May play a role in stabilization of EPO mRNA during hypoxic stress. Isoform Mxi2 activation is stimulated by mitogens and oxidative stress and only poorly phosphorylates ELK1 and ATF2. Isoform Exip may play a role in the early onset of apoptosis. |
| Immunogen | Peptide sequence around aa. 180~184 (T-G-Y-V-A) derived from Human P38 MAPK. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: , ICC/IF: . Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
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| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CSBP CSBP1 CSBP2 CSPB1 EXIP Mxi2 PRKM14 PRKM15 RK SAPK2A p38 p38ALPHA MAPK14 mitogen-activated protein kinase 14 Csaids binding protein MAP kinase Mxi2 cytokine suppressive anti-inflammatory drug-binding protein mitogen-activated protein k |
|---|---|
| Target | P38 MAPK |
| Molecular Weight(MW) | 43 (note) |
Application
Western blot analysis of extracts from 293 and Hela cells using P38 MAPK Antibody .
Application
Western blot analysis of extracts from 293 and Hela cells using P38 MAPK (Ab-182) Antibody| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: , ICC/IF: . Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
