Application
Western Blot of HeLa cells lysates using Anti-p38 MAPK RM245 at a 1:1000 dilution, showed a band of p38 MAPK (~38 kDa) expressed in HeLa cells.Application
Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer tissuesections using Anti-p38 MAPK RM245 at a 1:200 dilution.| Product Name | p38 MAPK antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Monoclonal antibody [RM245] to p38 MAPKThe protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008] |
| Immunogen | A peptide corresponding to human p38 MAPK. |
| Modification | Acetylated |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | RM245 |
| Host Species | Rabbit |
| Tested Applications | IHCIHC-PWB |
| WB:1:500~1:2000 IHC-P:1:50~1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | p38ALPHA SAPK2A EXIP Mxi2 mitogen-activated protein kinase 14 p38 MAPK14 CSBP RK CSPB1 PRKM15 CSBP2 PRKM14 CSBP1 |
|---|---|
| Target | p38 MAPK |
| Tissue Specificity | This antibody reacts to human p38 MAPK. This antibody may also react to rat and mouse p38 MAPK, as predicted by immunogen homology. |
Application
Western Blot of HeLa cells lysates using Anti-p38 MAPK RM245 at a 1:1000 dilution, showed a band of p38 MAPK (~38 kDa) expressed in HeLa cells.
Application
Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer tissuesections using Anti-p38 MAPK RM245 at a 1:200 dilution.| Application Notes | WB:1:500~1:2000 IHC-P:1:50~1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20.0°C |
| Storage Buffer | 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
