Application
Sample (30 ug of whole cell lysate) A: H1299 7.5% SDS PAGE diluted at 1:1000| Product Name | P4HA2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to P4HA2 (procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide II)This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human P4HA2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Chicken, Rhesus Monkey, BovineMouse (93%), Rat (93%), Chicken (81%), Rhesus Monkey (98%), Bovine (94%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | P4HA2 antibody C-P4Halpha(II) antibody 4-PH alpha-2 antibody prolyl 4-hydroxylase subunit alpha-2 antibody "procollagen-proline 2-oxoglutarate-4-dioxygenase subunit alpha-2 antibody" collagen prolyl 4-hydroxylase alpha(II) antibody "procollagen-proli |
|---|---|
| Target | P4HA2 |
| Molecular Weight(MW) | 61 kDa (note) (2) |
| Cellular Localization | Endoplasmic reticulum lumen |
Application
Sample (30 ug of whole cell lysate) A: H1299 7.5% SDS PAGE diluted at 1:1000| Positive Control | H1299 , HeLa |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20oC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
