Application
Immunofluorescence analysis of HeLa cells, using p53 (Acetyl-Lys386) Antibody. The picture on the right is treated with the synthesized peptide.Application
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using p53 (Acetyl-Lys386) Antibody. The picture on the right is treated with the synthesized peptide.| Product Name | p53 (acetyl Lys386) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to p53 (acetyl Lys386)Regulator that plays a central role in regulation of apoptosis and cell growth via its interactions. Regulates TP53 by enhancing the DNA binding and transactivation function of TP53 on the promoters of proapoptotic genes in vivo. Inhibits the ability of APPBP1 to conjugate NEDD8 to CUL1, and thereby decreases APPBP1 ability to induce apoptosis. Impedes cell cycle progression at G2/M. Its apoptosis-stimulating activity is inhibited by its interaction with DDX42. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human p53 around the acetylated site of Lys386. |
| Modification | acetyl Lys386 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFIHCIHC-P |
| IF: 1:100~1:500 : |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Antigen NYCO13 Cellular tumor antigen p53 Tumor suppressor p53 Phosphoprotein p53 Antigen NY-CO-13 TP53 p53 Antigen NY CO 13 p53 (acetyl K386) p53 (acetyl Lys386) p53 acetyl K386 p53 acetyl Lys386 Acetyl p53 (K386) Acetyl p53 (Lys386) |
|---|---|
| Target | p53 (acetyl Lys386) |
| Molecular Weight(MW) | 43 kDa (note) |
| Tissue Specificity | p53 (Acetyl-Lys386) Antibody detects endogenous levels of total p53 protein only when acetylated at lysine386. |
| Cellular Localization | Predominantly found in the perinuclear region. Some small fraction is nuclear. Sequester in the cytoplasm on overexpression of DDX42. |
Application
Immunofluorescence analysis of HeLa cells, using p53 (Acetyl-Lys386) Antibody. The picture on the right is treated with the synthesized peptide.
Application
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using p53 (Acetyl-Lys386) Antibody. The picture on the right is treated with the synthesized peptide.| Application Notes | IF: 1:100~1:500 : |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 degrees Celcius. Product is stable for at least 1 year. |
| Storage Buffer | Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
