Application
WB analysis of Colon carcinoma whole cell extract (HCT116 WCE, 100 μg) using p53 (mono-Methyl Lys372) antibody at a dilution of 1:200 in TBS-Tween containing 5% skimmed milk.| Product Name | p53 (mono-methyl Lys372) antibody - ChIP grade |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to p53 (mono-methyl Lys372)This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons (PMIDs: 12032546, 20937277). [provided by RefSeq, Feb 2013] |
| Immunogen | Polyclonal antibody raised in rabbit against human p53 (tumor protein p53), methylated at K372, using a KLH-conjugated synthetic peptide. |
| Modification | mono-methyl Lys372 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WBChIP |
| Western blotting (1:200), ChIP (5 µg/ChIP): |
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| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Synonyms | tumor protein p53 P53 BCC7 TRP53 LFS1 TP53 |
|---|---|
| Target | p53 (mono-methyl Lys372) |
Application
WB analysis of Colon carcinoma whole cell extract (HCT116 WCE, 100 μg) using p53 (mono-Methyl Lys372) antibody at a dilution of 1:200 in TBS-Tween containing 5% skimmed milk.| Application Notes | Western blotting (1:200), ChIP (5 µg/ChIP): |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS containing 0.05% azide and 0.05% ProClin 300. |
