Application
Western blot analysis of p53 (pS33) expression in MCF7 Actinomycin-treated (A); Jurkat (B) whole cell lysates.| Product Name | p53 (phospho Ser33) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to p53 (phospho Ser33)This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons (PMIDs: 12032546, 20937277). [provided by RefSeq, Feb 2013] |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human p53 (pS33). The exact sequence is proprietary. |
| Modification | p-Ser33 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: 1:500 - 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | TP53 P53 TRP53 LFS1 BCC7 tumor protein p53 |
|---|---|
| Target | p53 (phospho Ser33) |
| Tissue Specificity | Recognizes endogenous levels of p53 (pS33) protein. |
Application
Western blot analysis of p53 (pS33) expression in MCF7 Actinomycin-treated (A); Jurkat (B) whole cell lysates.| Application Notes | WB: 1:500 - 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4 °C. Upon delivery aliquot and store at -20 °C for one year. Avoid freeze/thaw cycles. |
| Storage Buffer | Liquid in 0.42% Potassium phosphate; 0.87% Sodium chloride; pH 7.3; 30% glycerol; and 0.01% sodium azide. |
