Application
Immunofluorescence staining of methanol-fixed Hela cells using p53 (Phospho-Ser33) Antibody .Application
Western blot analysis of extracts from HT29 cells using p53 (Phospho-Ser33) Antibody and the same antibody preincubated with blocking peptide.| Product Name | p53 (phospho Ser33) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to p53 (Phospho Ser33)Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. Implicated in Notch signaling cross-over. |
| Immunogen | Peptide sequence around phosphorylation site of serine 33 (V-L-S(p)-P-L) derived from Human p53. |
| Modification | p-Ser33 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | FLJ92943 LFS1 P53 TRP53 TP53 cellular tumor antigen p53 antigen NY-CO-13 phosphoprotein p53 p53 tumor suppressor transformation-related protein 53 tumor protein p53 |
|---|---|
| Target | p53 (Phospho Ser33) |
| Molecular Weight(MW) | 53 (note) |
Application
Immunofluorescence staining of methanol-fixed Hela cells using p53 (Phospho-Ser33) Antibody .
Application
Western blot analysis of extracts from HT29 cells using p53 (Phospho-Ser33) Antibody and the same antibody preincubated with blocking peptide.| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
