Application
ICC/IF analysis of methanol-fixed HeLa cells using p53 (phospho Ser37) antibody.Application
WB analysis of extract from HT-29 cells, untreated or treated with Doxorubicin (1mM, 30 min), using p53 antibody (Lane 1 and 2) and p53 (phospho Ser37) antibody (Lane 3 and 4).| Product Name | p53 (phospho Ser37) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to p53 (phospho Ser37)This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons (PMIDs: 12032546, 20937277). [provided by RefSeq, Feb 2013] |
| Immunogen | The antiserum was produced against synthesized phosphopeptide derived from human p53 around the phosphorylation site of serine 37 (L -P-SP-Q-A). |
| Modification | p-Ser37 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB: 1:500-1:1000 IF:1:100-1:200: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | BCC7 TRP53 LFS1 tumor protein p53 P53 TP53 |
|---|---|
| Target | p53 (phospho Ser37) |
| Tissue Specificity | p53 (phospho-Ser37) antibody detects endogenous levels of p53 only when phosphorylated at serine 37. |
Application
ICC/IF analysis of methanol-fixed HeLa cells using p53 (phospho Ser37) antibody.
Application
WB analysis of extract from HT-29 cells, untreated or treated with Doxorubicin (1mM, 30 min), using p53 antibody (Lane 1 and 2) and p53 (phospho Ser37) antibody (Lane 3 and 4).| Application Notes | WB: 1:500-1:1000 IF:1:100-1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C or -80°C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
