Application
Western blot analysis of extracts from 293 cells, treated with UV+serum or calf intestinal phosphatase (CIP), using p53 (Phospho-Ser46) Antibody.Application
Western blot analysis of extracts from MCF cells untreated or treated with Anisomycin using p53(Phospho-Ser46) Antibody.Application
Immunofluorescence staining of methanol-fixed Hela cells using p53 (Phospho-Ser46) Antibody .Application
Western blot analysis of extracts from MCF cells untreated or treated with Anisomycin using p53 (Phospho-Ser46) Antibody .| Product Name | p53 (phospho Ser46) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to p53 (Phospho Ser46)p53 is a nuclear protein which plays an essential role in the regulation of cell cycle specifically in the transition from G0 to G1. It is found in very low levels in normal cells however in a variety of transformed cell lines in high amounts and believed to contribute to transformation and malignancy. The open reading frame of p53 is 393 amino acids long, with the central region (consisting of amino acids from about 100 to 300) containing the DNA-binding domain. This proteolysis-resistant core is flanked by a C-terminal end mediating oligomerization and an N-terminal end containing a strong transcription activation signal. p53 binds as a tetramer to a PBS (p53-Binding Site) and activates the expression of downstream genes that inhibit growth and/or invasion. p53 binds as a tetramer to a p53-binding site (PBS) and to activate the expression of adjacent genes that inhibit growth and/or invasion. Deletion of one or both p53 alleles reduces the expression of tetramers, resulting in decreased expression of the growth inhibitory genes |
| Immunogen | Peptide sequence around phosphorylation site of serine 46 (M-L-S(p)-P-D) derived from Human p53. |
| Modification | p-Ser46 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | FLJ92943 LFS1 P53 TRP53 TP53 cellular tumor antigen p53 antigen NY-CO-13 phosphoprotein p53 p53 tumor suppressor transformation-related protein 53 tumor protein p53 |
|---|---|
| Target | p53 (Phospho Ser46) |
| Molecular Weight(MW) | 53 (note) |
Application
Western blot analysis of extracts from 293 cells, treated with UV+serum or calf intestinal phosphatase (CIP), using p53 (Phospho-Ser46) Antibody.
Application
Western blot analysis of extracts from MCF cells untreated or treated with Anisomycin using p53(Phospho-Ser46) Antibody.
Application
Immunofluorescence staining of methanol-fixed Hela cells using p53 (Phospho-Ser46) Antibody .
Application
Western blot analysis of extracts from MCF cells untreated or treated with Anisomycin using p53 (Phospho-Ser46) Antibody .| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
