Application
Immunofluorescence staining of methanol-fixed Hela cells using p53 Antibody .Application
Western blot analysis of extracts from MDA and JK cells using p53(Ab-15) AntibodyApplication
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p53 Antibody (left) or the same antibody preincubated with blocking peptide (right).| Product Name | p53 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to p53p53 is a nuclear protein which plays an essential role in the regulation of cell cycle specifically in the transition from G0 to G1. It is found in very low levels in normal cells however in a variety of transformed cell lines in high amounts and believed to contribute to transformation and malignancy. The open reading frame of p53 is 393 amino acids long, with the central region (consisting of amino acids from about 100 to 300) containing the DNA-binding domain. This proteolysis-resistant core is flanked by a C-terminal end mediating oligomerization and an N-terminal end containing a strong transcription activation signal. p53 binds as a tetramer to a PBS (p53-Binding Site) and activates the expression of downstream genes that inhibit growth and/or invasion. p53 binds as a tetramer to a p53-binding site (PBS) and to activate the expression of adjacent genes that inhibit growth and/or invasion. Deletion of one or both p53 alleles reduces the expression of tetramers, resulting in decreased expression of the growth inhibitory genes |
| Immunogen | Peptide sequence around aa.13~17 (P-L-S-Q-E) derived from Human p53. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | FLJ92943 LFS1 P53 TRP53 TP53 cellular tumor antigen p53 antigen NY-CO-13 phosphoprotein p53 p53 tumor suppressor transformation-related protein 53 tumor protein p53 |
|---|---|
| Target | p53 |
| Molecular Weight(MW) | 53 (note) |
Application
Immunofluorescence staining of methanol-fixed Hela cells using p53 Antibody .
Application
Western blot analysis of extracts from MDA and JK cells using p53(Ab-15) Antibody
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p53 Antibody (left) or the same antibody preincubated with blocking peptide (right).| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
