Application
p54/nrb antibody detects p54/nrb protein at nucleus in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. p54/nrb antibody diluted at 1:500.Application
p54/nrb antibody detects p54/nrb protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: p54/nrb protein stained by p54/nrb antibody diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000. Blue: Hoechst 33342 staining.Application
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using p54/nrb antibody at 1:100 dilution.Application
Sample (30 ug of whole cell lysate) A: Hep G2 10% SDS PAGE diluted at 1:1000| Product Name | p54/nrb antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to p54/nrb (non-POU domain containing, octamer-binding)This gene encodes an RNA-binding protein which plays various roles in the nucleus, including transcriptional regulation and RNA splicing. A rearrangement between this gene and the transcription factor E3 gene has been observed in papillary renal cell carcinoma. Alternatively spliced transcript variants have been described. Pseudogenes exist on Chromosomes 2 and 16. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human p54/nrb. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Xenopus laevis, Chicken, Chimpanzee, BovineRat (100%), Xenopus laevis (86%), Chicken (98%), Chimpanzee (100%), Bovine (99%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | NMT55 antibody NRB54 antibody P54 antibody P54NRB antibody NONO antibody "DNA-binding p52/p100 complex 52 kDa subunit antibody" non-POU domain-containing octamer (ATGCAAAT) binding protein antibody p54(nrb) antibody 55 kDa nuclear protein antibody |
|---|---|
| Target | p54/nrb |
| Molecular Weight(MW) | 54 kDa (note) (3) |
| Cellular Localization | Nucleus |
Application
p54/nrb antibody detects p54/nrb protein at nucleus in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. p54/nrb antibody diluted at 1:500.
Application
p54/nrb antibody detects p54/nrb protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: p54/nrb protein stained by p54/nrb antibody diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000. Blue: Hoechst 33342 staining.
Application
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using p54/nrb antibody at 1:100 dilution.
Application
Sample (30 ug of whole cell lysate) A: Hep G2 10% SDS PAGE diluted at 1:1000| Positive Control | 293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
