Application
Western blot analysis of extracts from Jurkat cells using p62Dok(Ab-362) Antibody and the same antibody preincubated with blocking peptide.Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p62Dok Antibody (left) or the same antibody preincubated with blocking peptide (right).Application
Western blot analysis of extracts from Jurkat cells using p62Dok Antibody and the same antibody preincubated with blocking peptide.| Product Name | p62Dok antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to p62DokDOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK1 appears to be a negative regulator of the insulin signaling pathway. Modulates integrin activation by competing with talin for the same binding site on ITGB3. |
| Immunogen | Peptide sequence around aa.360~364 (P-I-Y-D-E) derived from Human p62Dok. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | MGC117395 MGC138860 P62DOK DOK1 Downstream of tyrosine kinase 1 docking protein 1 (downstream of tyrosine kinase 1) pp62 docking protein 1 p62(dok) docking protein 1 62kDa (downstream of tyrosine kinase 1) |
|---|---|
| Target | p62Dok |
| Molecular Weight(MW) | 62 (note) |
Application
Western blot analysis of extracts from Jurkat cells using p62Dok(Ab-362) Antibody and the same antibody preincubated with blocking peptide.
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p62Dok Antibody (left) or the same antibody preincubated with blocking peptide (right).
Application
Western blot analysis of extracts from Jurkat cells using p62Dok Antibody and the same antibody preincubated with blocking peptide.| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
