Application
Immunofluorescence staining of methanol-fixed Hela cells using p62Dok Antibody .Application
Western blot analysis of extracts from K562, HL-60 and JK cells using p62Dok(Ab-398) AntibodyApplication
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p62Dok Antibody (left) or the same antibody preincubated with blocking peptide (right).| Product Name | p62Dok antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to p62DokDOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK1 appears to be a negative regulator of the insulin signaling pathway. Modulates integrin activation by competing with talin for the same binding site on ITGB3. |
| Immunogen | Peptide sequence around aa.396~400 (P-I-Y-D-E) derived from Human p62Dok. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | MGC117395 MGC138860 P62DOK DOK1 Downstream of tyrosine kinase 1 docking protein 1 (downstream of tyrosine kinase 1) pp62 docking protein 1 p62(dok) docking protein 1 62kDa (downstream of tyrosine kinase 1) |
|---|---|
| Target | p62Dok |
| Molecular Weight(MW) | 62 (note) |
Application
Immunofluorescence staining of methanol-fixed Hela cells using p62Dok Antibody .
Application
Western blot analysis of extracts from K562, HL-60 and JK cells using p62Dok(Ab-398) Antibody
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p62Dok Antibody (left) or the same antibody preincubated with blocking peptide (right).| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
