Application
Immunofluorescence staining of methanol-fixed MCF cells using p70 S6 Kinase (Phospho-Thr421) Antibody .Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p70 S6 Kinase (Phospho-Thr421) Antibody (left) or the same antibody preincubated with blocking peptide (right).Application
Western blot analysis of extracts from Jurkat cells untreated (lane 1) or treated with GM-CSF (lane 2) using p70 S6 Kinase (Phospho-Thr421) Antibody .| Product Name | p70 S6K (phospho Thr421) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to p70 S6K (phospho Thr421)Phosphorylates specifically ribosomal protein S6 in response to insulin or several classes of mitogens. Promotes protein synthesis by phosphorylating PDCD4 at 'Ser-67' and targeting it for degradation. |
| Immunogen | Peptide sequence around phosphorylation site of threonine 421 (P-R-T(p)-P-V) derived from Human p70 S6 Kinase. |
| Modification | p-Thr421 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | PS6K S6K S6K1 STK14A p70(S6K)-alpha p70-S6K p70-alpha RPS6KB1 ribosomal protein S6 kinase I p70 S6 kinase alpha 1 serine/threonine kinase 14 alpha serine/threonine-protein kinase 14A p70 S6KA P70S6K1 70 kDa ribosomal protein S6 kinase 1 p7 |
|---|---|
| Target | p70 S6K (phospho Thr421) |
| Molecular Weight(MW) | 70, 85 (note) |
Application
Immunofluorescence staining of methanol-fixed MCF cells using p70 S6 Kinase (Phospho-Thr421) Antibody .
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p70 S6 Kinase (Phospho-Thr421) Antibody (left) or the same antibody preincubated with blocking peptide (right).
Application
Western blot analysis of extracts from Jurkat cells untreated (lane 1) or treated with GM-CSF (lane 2) using p70 S6 Kinase (Phospho-Thr421) Antibody .| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
