Application
A. Western blot analysis on 293T cell lysate using anti-Phospho-p70 S6 Kinase (pT421/pS424) RabMAb (cat. #); dilution 1:2500. Cell lysates were either (1) untreated or (2) treated with EGF.| Product Name | p70 S6K (phospho Thr421/Ser424) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit monoclonal antibody [E135] to p70 S6K (phospho Thr421/Ser424)p70 S6 Kinase is a mitogen-activated Ser/Thr protein kinase that is required for cell growth and G1 cell cycle progression . p70 S6 kinase phosphorylates specifically ribosomal protein S6. Activation of p70 S6 kinase is controlled by multiple phosphorylation events located within the catalytic, linker and pseudosubstrate domains . Activation occurs via phosphorylation at Ser411, Thr421 and Ser424 within the pseudosubstrate region . |
| Immunogen | A synthetic phospho-peptide corresponding to residues surrounding Thr421 and Ser424 of human p70 S6 Kinase alpha was used as immunogen. |
| Modification | p-Thr421/Ser424 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | E135 |
| Host Species | Rabbit |
| Tested Applications | IPWB |
| For WB: Use at a dilution of 1:2,500: |
|
| Species Reactivity | HumanRatFish |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | p70-alpha p70-S6K p70S6K p70alpha p70(S6K)alpha STK14A RPS6KB1 S6K1 p70(S6K)-alpha PS6K S6K p70 S6K p70 alpha p70(S6K) alpha p70 S6 Kinase (phospho T421/S424) p70 S6 Kinase (phospho Thr421/Ser424) p70 S6 Kinase (pT421/pS424) p70 S6 Kinase |
|---|---|
| Target | p70 S6K (phospho Thr421/Ser424) |
| Molecular Weight(MW) | 70 (note) |
| Tissue Specificity | The antibody only detects p70-S6K phosphorylated on Threonine 421 and Serine 424. |
Application
A. Western blot analysis on 293T cell lysate using anti-Phospho-p70 S6 Kinase (pT421/pS424) RabMAb (cat. #); dilution 1:2500. Cell lysates were either (1) untreated or (2) treated with EGF.| Application Notes | For WB: Use at a dilution of 1:2,500: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 °C. Stable for 12 months from date of receipt. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
