Application
Western blot analysis of S6K1 (pT444) expression in HepG2 EGF-treated (A); mouse brain (B); rat brain (C) whole cell lysates.Application
Immunofluorescent analysis of S6K1 (pT444) staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).Application
Immunohistochemical analysis of S6K1 (pT444) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.WB
TAC operation for 2 weeks markedly induced activation of AKT, mTOR, S6K, and ERK1/2 and inhibition of AMPK activation compared with sham controls, but this effect was reversed by RES in TAC-treated mice (D).| Product Name | p70 S6K (phospho Thr444) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to p70 S6K (phospho Thr444)This gene encodes a member of the ribosomal S6 kinase family of serine/threonine kinases. The encoded protein responds to mTOR (mammalian target of rapamycin) signaling to promote protein synthesis, cell growth, and cell proliferation. Activity of this gene has been associated with human cancer. Alternatively spliced transcript variants have been observed. The use of alternative translation start sites results in isoforms with longer or shorter N-termini which may differ in their subcellular localizations. There are two pseudogenes for this gene on chromosome 17. [provided by RefSeq, Jan 2013] |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human S6K1 (pT444). The exact sequence is proprietary. |
| Modification | p-Thr444 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| WB: 1:500 - 1:1000. IHC-P: 1:100 - 1:200. ICC/IF: 1:100 - 1:500: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | RPS6KB1 STK14A p70 S6KA S6K-beta-1 S6K p70-alpha ribosomal protein S6 kinase 70kDa polypeptide 1 p70-S6K S6K1 PS6K p70(S6K)-alpha |
|---|---|
| Target | p70 S6K (phospho Thr444) |
| Tissue Specificity | Recognizes endogenous levels of S6K1 (pT444) protein. |
Application
Western blot analysis of S6K1 (pT444) expression in HepG2 EGF-treated (A); mouse brain (B); rat brain (C) whole cell lysates.
Application
Immunofluorescent analysis of S6K1 (pT444) staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Application
Immunohistochemical analysis of S6K1 (pT444) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
WB
TAC operation for 2 weeks markedly induced activation of AKT, mTOR, S6K, and ERK1/2 and inhibition of AMPK activation compared with sham controls, but this effect was reversed by RES in TAC-treated mice (D).| Application Notes | WB: 1:500 - 1:1000. IHC-P: 1:100 - 1:200. ICC/IF: 1:100 - 1:500: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4 °C. Upon delivery aliquot and store at -20 °C for one year. Avoid freeze/thaw cycles. |
| Storage Buffer | Liquid in 0.42% Potassium phosphate; 0.87% Sodium chloride; pH 7.3; 30% glycerol; and 0.01% sodium azide. |
