Application
Western blot analysis of extracts from 293 cells using p70 S6 Kinase(Ab-421) Antibody (Lane 2) and the same antibody preincubated with blocking peptide (Lane 1)Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p70 S6 Kinase Antibody (left) or the same antibody preincubated with blocking peptide (right).Application
Western blot analysis of extracts from 293 cells using p70 S6 Kinase Antibody (Lane 2) and the same antibody preincubated with blocking peptide (Lane1).| Product Name | p70 S6K antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to p70 S6KPhosphorylates specifically ribosomal protein S6 in response to insulin or several classes of mitogens. Promotes protein synthesis by phosphorylating PDCD4 at 'Ser-67' and targeting it for degradation. |
| Immunogen | Peptide sequence around aa.419~423 (P-R-T-P-V) derived from Human p70 S6 Kinase. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | PS6K S6K S6K1 STK14A p70(S6K)-alpha p70-S6K p70-alpha RPS6KB1 ribosomal protein S6 kinase I p70 S6 kinase alpha 1 serine/threonine kinase 14 alpha serine/threonine-protein kinase 14A p70 S6KA P70S6K1 70 kDa ribosomal protein S6 kinase 1 p7 |
|---|---|
| Target | p70 S6K |
| Molecular Weight(MW) | 70, 85 (note) |
Application
Western blot analysis of extracts from 293 cells using p70 S6 Kinase(Ab-421) Antibody (Lane 2) and the same antibody preincubated with blocking peptide (Lane 1)
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p70 S6 Kinase Antibody (left) or the same antibody preincubated with blocking peptide (right).
Application
Western blot analysis of extracts from 293 cells using p70 S6 Kinase Antibody (Lane 2) and the same antibody preincubated with blocking peptide (Lane1).| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
