Application
S6K antibody detects S6K protein by western blot analysis. Whole cell extracts (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with S6K antibody at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with p70 S6K antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with p70 S6K antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using p70 S6K antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.Application
Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using p70 S6K antibody at 1:100 dilution.Application
Sample (30 µg of whole cell lysate) A: A431 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
p70 S6K antibody detects RPS6KB1 protein at cytoplasm in rat kidney by immunohistochemical analysis. Sample: Paraffin-embedded rat kidney. p70 S6K antibody diluted at 1:500.| Product Name | p70 S6K antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to S6K (ribosomal protein S6 kinase, 70kDa, polypeptide 1)This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates several residues of the S6 ribosomal protein. The kinase activity of this protein leads to an increase in protein synthesis and cell proliferation. Amplification of the region of DNA encoding this gene and overexpression of this kinase are seen in some breast cancer cell lines. Alternate translational start sites have been described and alternate transcriptional splice variants have been observed but have not been thoroughly characterized. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human p70 S6K. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Xenopus laevis, Rabbit, Chicken, BovineXenopus laevis (87%), Rabbit (100%), Chicken (92%), Bovine (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | PS6K antibody S6K antibody S6K1 antibody STK14A antibody p70(S6K)-alpha antibody p70-S6K antibody p70-alpha antibody RPS6KB1 antibody ribosomal protein S6 kinase I antibody "p70 S6 kinase alpha 1 antibody" serine/threonine kinase 14 alpha antibo |
|---|---|
| Molecular Weight(MW) | 59 kDa (note) (4) |
| Cellular Localization | Cytoplasm (By similarity) , Cell junction , synapse , synaptosome |
Application
S6K antibody detects S6K protein by western blot analysis. Whole cell extracts (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with S6K antibody at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with p70 S6K antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with p70 S6K antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using p70 S6K antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.
Application
Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using p70 S6K antibody at 1:100 dilution.
Application
Sample (30 µg of whole cell lysate) A: A431 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
p70 S6K antibody detects RPS6KB1 protein at cytoplasm in rat kidney by immunohistochemical analysis. Sample: Paraffin-embedded rat kidney. p70 S6K antibody diluted at 1:500.| Positive Control | 293T , A431 , Jurkat , Raji , HeLa |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
