Application
RSK1 [pS221] phosphospecific antibody. RSK1 [pS221] phosphospecific antibody A431 cells unstimulated (1) or PMA-stimulated were incubated with anti-RSK1 [pS221] after prior incubation with: no peptide (1, 2), the non-phosphopeptide (3), a generic [pS] peptide (4), or, the phosphopeptide immunogen (5). Data show RSK phosphorylation by PMA treatment and also demonstrate phospho-antibody specificity by peptide competition| Product Name | p90 RSK1 (phospho Ser221) antibody |
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| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to p90 RSK1 (phospho Ser221)RSK1 (also called MAPK Activated Protein Kinase-1a; MAPKAP-K1a) is a member of the broadly expressed p90 Ribosomal S6 Kinase (RSK) family of serine/threonine kinases that also includes RSK2 (MAPKAP-K1b), RSK3 (MAPKAP-K1c), and RSK4. RSK proteins are substrates for and downstream transducers of MAPK signaling proteins, primarily ERK1&2. RSK proteins possess two separate kinase domains, the C-terminal kinase domain and the N-terminal kinase domain, separated by a linker region containing a hydrophobic motif. Activation of RSK1 is a multi-step process involving phosphorylation of multiple residues within the three domains, changes in RSK protein localization, and modulation of RSK complex formation with ERK1&2 and/or 14-3-3 beta proteins. RSK is activated by many stimuli including growth factors, phorbol esters, cAMP, heat shock, and irradiation. ERK 1&2 phosphorylate threonine 573 of the C-terminal kinase domain, thereby stimulating this kinase activity and leading to phosphorylation of serine 380 within the linker region. Phosphoserine 380 is part of a docking site for PDK1, a constitutively active cytoplasmic kinase that phosphorylates serine 221 and thereby activates the N-terminal kinase domain to phosphorylate various substrates that include transporters, transcription factors, and transcription co-activators. |
| Immunogen | Synthetic peptide (Human). |
| Modification | p-Ser221 |
| Clonality | Polyclonal |
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| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: Use at a dilution of 1/1000. Predicted molecular weight: 90 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
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| Species Reactivity | MouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | HU-1 HU1 6195 Q15418 RPS6KA1 MAPKAPK1A S6Kalpha 1 S6Kalpha1 RSK RSK1 p90 S6K-alpha 1 601684 RSK1 HU 1 S6K alpha 1 RSK1 p90 (phospho S221) RSK1 p90 (phospho Ser221) RSK1 p90 (pS221) RSK1 p90 (pSer221) RSK1 p90 phospho S221 RSK1 p90 phosp |
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| Target | p90 RSK1 (phospho Ser221) |
Application
RSK1 [pS221] phosphospecific antibody. RSK1 [pS221] phosphospecific antibody A431 cells unstimulated (1) or PMA-stimulated were incubated with anti-RSK1 [pS221] after prior incubation with: no peptide (1, 2), the non-phosphopeptide (3), a generic [pS] peptide (4), or, the phosphopeptide immunogen (5). Data show RSK phosphorylation by PMA treatment and also demonstrate phospho-antibody specificity by peptide competition| Positive Control | A431 and NIH3T3 cells treated with PMA, a phorbol ester activator of protein kinase C |
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| Application Notes | WB: Use at a dilution of 1/1000. Predicted molecular weight: 90 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
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| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | PBS pH 7.3 (without Mg2+ and Ca2+) containing 50% glycerol, 1.0mg/ml BSA (IgG, protease free). and 0.05% sodium azide. |
