Application
Lysates prepared from A431 cells left unstimulated (1) or stimulated with 100 ng/mL PMA (a phorbol ester activator of protein kinase C) for 30 minutes at 37oC (2-5) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer for one hour at room temperature and incubated with RSK1 [pS380]/2 [pS386] antibody for one hour at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1, 2), the non-phosphopeptide corresponding to the immunogen (3), a generic phosphoserine-containing peptide (4), or, the phosphopeptide immunogen (5). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG HRP conjugate and bands were detected. The data show induction of the RSK1 [pS380]/2 [pS386] antibody signal by PMA treatment and that the peptide corresponding to this site blocks the antibody signal, thereby demonstrating the specificity of the antibody.| Product Name | p90 RSK1 (phospho Ser380) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to p90 RSK1 (phospho Ser380)RSK1 (also called MAPK Activated Protein Kinase-1a; MAPKAP-K1a) is a member of the broadly expressed p90 Ribosomal S6 Kinase (RSK) family of serine/threonine kinases that also includes RSK2 (MAPKAP-K1b), RSK3 (MAPKAP-K1c), and RSK4. RSK proteins are substrates for and downstream transducers of MAPK signaling proteins, primarily ERK1&2. RSK proteins possess two separate kinase domains, the C-terminal kinase domain and the N-terminal kinase domain, separated by a linker region containing a hydrophobic motif. Activation of RSK1 is a multi-step process involving phosphorylation of multiple residues within the three domains, changes in RSK protein localization, and modulation of RSK complex formation with ERK1&2 and/or 14-3-3 beta proteins. RSK is activated by many stimuli including growth factors, phorbol esters, cAMP, heat shock, and irradiation. ERK 1&2 phosphorylates threonine 573 of the C-terminal kinase domain, thereby stimulating this kinase activity and leading to phosphorylation of serine 380 within the linker region. Phosphoserine 380 is part of a docking site for PDK1, a constitutively active cytoplasmic kinase that phosphorylates serine 221 and thereby activates the N-terminal kinase domain to phosphorylate various substrates that include transporters, transcription factors, and transcription co-activators. |
| Immunogen | Synthetic peptide (Human). |
| Modification | p-Ser380 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: Use at a dilution of 1/1000. Predicted molecular weight: 90 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | HU-1 HU1 6195 Q15418 RPS6KA1 MAPKAPK1A S6Kalpha 1 S6Kalpha1 RSK RSK1 p90 S6K-alpha 1 601684 RSK1 HU 1 S6K alpha 1 RSK1 p90 (phospho S380) RSK1 p90 (phospho Ser380) RSK1 p90 (pS380) RSK1 p90 (pSer380) RSK1 p90 phospho S380 RSK1 p90 phosp |
|---|---|
| Target | p90 RSK1 (phospho Ser380) |
Application
Lysates prepared from A431 cells left unstimulated (1) or stimulated with 100 ng/mL PMA (a phorbol ester activator of protein kinase C) for 30 minutes at 37oC (2-5) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer for one hour at room temperature and incubated with RSK1 [pS380]/2 [pS386] antibody for one hour at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1, 2), the non-phosphopeptide corresponding to the immunogen (3), a generic phosphoserine-containing peptide (4), or, the phosphopeptide immunogen (5). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG HRP conjugate and bands were detected. The data show induction of the RSK1 [pS380]/2 [pS386] antibody signal by PMA treatment and that the peptide corresponding to this site blocks the antibody signal, thereby demonstrating the specificity of the antibody.| Positive Control | A431 and NIH3T3 cells treated with PMA, a phorbol ester activator of protein kinase C |
|---|---|
| Application Notes | WB: Use at a dilution of 1/1000. Predicted molecular weight: 90 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | PBS pH 7.3 (without Mg2+ and Ca2+) containing 50% glycerol, 1.0mg/ml BSA (IgG, protease free). and 0.05% sodium azide. |
