Application
Sample (30 ug of whole cell lysate) A: Hep G2 7.5% SDS PAGE diluted at 1:10000Application
Immunofluorescence analysis of methanol-fixed HeLa, using p90 RSK1 antibody at 1:200 dilution.Application
Immunohistochemical analysis of paraffin-embedded SCM1 xenograft, using p90 RSK1 antibody at 1:500 dilution.| Product Name | p90 RSK1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to RSK1 (ribosomal protein S6 kinase, 90kDa, polypeptide 1)This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 nonidentical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human p90 RSK1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Xenopus laevis, Pig, Chicken, BovineMouse (99%), Rat (99%), Xenopus laevis (97%), Pig (99%), Chicken (98%), Bovine (99%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | HU-1 antibody MAPKAPK1A antibody RSK antibody RSK1 antibody RPS6KA1 antibody MAPK-activated protein kinase 1a antibody MAPKAP kinase 1a antibody p90-RSK 1 antibody ribosomal S6 kinase 1 antibody ribosomal protein S6 kinase alpha 1 antibody "dJ590 |
|---|---|
| Molecular Weight(MW) | 83 kDa (note) (3) |
Application
Sample (30 ug of whole cell lysate) A: Hep G2 7.5% SDS PAGE diluted at 1:10000
Application
Immunofluorescence analysis of methanol-fixed HeLa, using p90 RSK1 antibody at 1:200 dilution.
Application
Immunohistochemical analysis of paraffin-embedded SCM1 xenograft, using p90 RSK1 antibody at 1:500 dilution.| Positive Control | A431 , HeLa , HepG2 , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
