Application
PABP antibody detects PABP protein at cytosol on mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. PABP antibody dilution: 1:500.Application
PABP antibody detects PABP protein at cytosol on mouse middle brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse middle brain. PABP antibody dilution: 1:500.Application
PABP antibody detects PABP protein at cytoplasm by immunofluorescent analysis. Sample: H1299 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PABP protein stained by PABP antibody diluted at 1:500. Blue: Hoechst 33342 staining.Application
PABP antibody detects PABP protein at cytosol on rat hind brain by immunohistochemical analysis. Sample: Paraffin-embedded rat hind brain. PABP antibody dilution: 1:500.Application
Sample (30 µg of whole cell lysate) A: A549 B: H1299 C: HCT116 D: MCF-7 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunohistochemical analysis of paraffin-embedded human hepatoma, using PABP antibody at 1:500 dilution.| Product Name | PABP antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PABP (polyadenylate-binding protein 1)The poly(A)-binding protein (PABP), which is found complexed to the 3-prime poly(A) tail of eukaryotic mRNA, is required for poly(A) shortening and translation initiation. In humans, the PABPs comprise a small nuclear isoform and a conserved gene family that displays at least 3 functional proteins: PABP1 (PABPC1), inducible PABP (iPABP, or PABPC4; MIM 603407), and PABP3 (PABPC3; MIM 604680). In addition, there are at least 4 pseudogenes, PABPCP1 to PABPCP4.[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PABP. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Xenopus laevis, Chicken, Rhesus Monkey, BovineXenopus laevis (91%), Chicken (95%), Rhesus Monkey (100%), Bovine (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | PAB1 antibody PABP antibody PABP1 antibody PABPC2 antibody PABPL1 antibody PABPC1 antibody polyadenylate-binding protein 1 antibody "poly(A) binding protein cytoplasmic 2 antibody" "poly(A) binding protein cytoplasmic 1 antibody" |
|---|---|
| Molecular Weight(MW) | 71 kDa (note) (2) |
| Cellular Localization | Cytoplasm , Nucleus |
Application
PABP antibody detects PABP protein at cytosol on mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. PABP antibody dilution: 1:500.
Application
PABP antibody detects PABP protein at cytosol on mouse middle brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse middle brain. PABP antibody dilution: 1:500.
Application
PABP antibody detects PABP protein at cytoplasm by immunofluorescent analysis. Sample: H1299 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PABP protein stained by PABP antibody diluted at 1:500. Blue: Hoechst 33342 staining.
Application
PABP antibody detects PABP protein at cytosol on rat hind brain by immunohistochemical analysis. Sample: Paraffin-embedded rat hind brain. PABP antibody dilution: 1:500.
Application
Sample (30 µg of whole cell lysate) A: A549 B: H1299 C: HCT116 D: MCF-7 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunohistochemical analysis of paraffin-embedded human hepatoma, using PABP antibody at 1:500 dilution.| Positive Control | A549 , H1299 , HCT116 , MCF-7 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
