Application
Sample (30 ug of whole cell lysate) A: U87-MG 10% SDS PAGE diluted at 1:1000Application
PABP5 antibody detects PABP5 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PABP5 antibody diluted by 1:1000.Application
Immunohistochemical analysis of paraffin-embedded human gastric cancer, using PABP5 antibody at 1:500 dilution.Application
PABP5 antibody detects PABP5 protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 10 % SDS-PAGE, and the membrane was blotted with PABP5 antibody diluted by 1:1000.Application
PABP5 antibody detects PABP5 protein by western blot analysis. Rat tissue extracts (50 μg) was separated by 10 % SDS-PAGE, and the membrane was blotted with PABP5 antibody diluted by 1:1000.| Product Name | PABP5 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PABP5 (poly(A) binding protein, cytoplasmic 5)This gene encodes a protein that binds to the polyA tail found at the 3' end of most eukaryotic mRNAs. It is thought to play a role in the regulation of mRNA metabolic processes in the cytoplasm. This gene is located in a gene-poor region within the X-specific 13d-sY43 subinterval of the chromosome Xq21.3/Yp11.2 homology block. It is located close to translocation breakpoints associated with premature ovarian failure, and is therefore a potential candidate gene for this disorder. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PABP5. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Rhesus Monkey, Chimpanzee, BovineRhesus Monkey (100%), Chimpanzee (100%), Bovine (95%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | FLJ51677 antibody PABP5 antibody PABPC5 antibody polyadenylate-binding protein 5 antibody "poly(A) binding protein cytoplasmic 5 antibody" |
|---|---|
| Molecular Weight(MW) | 43 kDa (note) (2) |
| Cellular Localization | Cytoplasm |
Application
Sample (30 ug of whole cell lysate) A: U87-MG 10% SDS PAGE diluted at 1:1000
Application
PABP5 antibody detects PABP5 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PABP5 antibody diluted by 1:1000.
Application
Immunohistochemical analysis of paraffin-embedded human gastric cancer, using PABP5 antibody at 1:500 dilution.
Application
PABP5 antibody detects PABP5 protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 10 % SDS-PAGE, and the membrane was blotted with PABP5 antibody diluted by 1:1000.
Application
PABP5 antibody detects PABP5 protein by western blot analysis. Rat tissue extracts (50 μg) was separated by 10 % SDS-PAGE, and the membrane was blotted with PABP5 antibody diluted by 1:1000.| Positive Control | A431 , HeLa , HepG2 , U87-MG , mouse brain , rat brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
