Application
Sample (30 µg of whole cell lysate) A: HepG2 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using PAD4 antibody at 1:200 dilution.Application
Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using PAD4 antibody at 1:500 dilution.| Product Name | PAD4 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PAD4 (peptidyl arginine deiminase, type IV)This gene is a member of a gene family which encodes enzymes responsible for the conversion of arginine residues to citrulline residues. This gene may play a role in granulocyte and macrophage development leading to inflammation and immune response. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human PAD4. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | PAD antibody PAD4 antibody PADI5 antibody PDI4 antibody PDI5 antibody PADI4 antibody "peptidyl arginine deiminase type V antibody" PADI-H protein antibody protein-arginine deiminase type IV antibody HL-60 PAD antibody protein-arginine deiminase |
|---|---|
| Molecular Weight(MW) | 74 kDa (note) (2) |
| Cellular Localization | Cytoplasm , Nucleus , Cytoplasmic granule |
Application
Sample (30 µg of whole cell lysate) A: HepG2 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using PAD4 antibody at 1:200 dilution.
Application
Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using PAD4 antibody at 1:500 dilution.| Positive Control | 293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
