Application
Sample (30 µg of whole cell lysate) A: A431 B: H1299 7.5% SDS PAGE diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using PAF1 antibody at 1:500 dilution.| Product Name | PAF1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to PAF1 (PAF1, RNA polymerase II associated factor, homolog (S. cerevisiae))PAF1, parafibromin (CDC73; MIM 607393), LEO1 (MIM 610507), and CTR9 (MIM 609366) form the PAF protein complex that associates with the RNA polymerase II subunit POLR2A (MIM 180660) and with a histone methyltransferase complex (Rozenblatt-Rosen et al., 2005 [PubMed 15632063]). The PAF complex also has a role in histone monoubiquitination (Zhu et al., 2005 [PubMed 16307923]).[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PAF1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Chimpanzee, BovineMouse (97%), Rat (96%), Chimpanzee (100%), Bovine (85%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | F23149_1 antibody FLJ11123 antibody PD2 antibody PAF1 antibody pancreatic differentiation protein 2 antibody hPAF1 antibody RNA polymerase II-associated factor 1 homolog antibody "Paf1 RNA polymerase II associated factor homolog (S. cerevisiae) an |
|---|---|
| Target | PAF1 |
| Molecular Weight(MW) | 60 kDa (note) |
| Cellular Localization | Nucleus |
Application
Sample (30 µg of whole cell lysate) A: A431 B: H1299 7.5% SDS PAGE diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using PAF1 antibody at 1:500 dilution.| Positive Control | 293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
