Application
Sample (30 µg of whole cell lysate) A: HepG2 10% SDS PAGE PAI-1 antibody diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunohistochemical analysis of paraffin-embedded human gastric cancer, using PAI1 antibody at 1:100 dilution.Application
Sample (30 µg of whole cell lysate) A:NIH-3T3 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | PAI-1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PAI-1 (serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1)This gene encodes a member of the serine proteinase inhibitor (serpin) superfamily. This member is the principal inhibitor of tissue plasminogen activator (tPA) and urokinase (uPA), and hence is an inhibitor of fibrinolysis. Defects in this gene are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency), and high concentrations of the gene product are associated with thrombophilia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PAI-1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Dog, Pig, Sheep, BovineRat (84%), Dog (89%), Pig (90%), Sheep (88%), Bovine (88%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | PAI antibody PAI-1 antibody PAI1 antibody PLANH1 antibody SERPINE1 antibody "serine (or cysteine) proteinase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1 antibody" serpin E1 antibody endothelial plasminogen activator |
|---|---|
| Molecular Weight(MW) | 45 kDa (note) (2) |
| Cellular Localization | Secreted |
Application
Sample (30 µg of whole cell lysate) A: HepG2 10% SDS PAGE PAI-1 antibody diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunohistochemical analysis of paraffin-embedded human gastric cancer, using PAI1 antibody at 1:100 dilution.
Application
Sample (30 µg of whole cell lysate) A:NIH-3T3 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | 293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , NIH-3T3 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
